| ACQ4 Neurophysiology Acquisition and Analysis System |
| Electrophysiology - Photostimulation - Fluorescence Imaging |
ACQ4 is a python-based platform for experimental neurophysiology
developed at the Allen Institute for Brain Science and the University of North Carolina, Chapel Hill. It includes support for patch-clamp electrophysiology, multiphoton imaging, scanning laser photostimulation, and many other experimental techniques. ACQ4 is highly modular and extensible, allowing support to be added for new types of devices, techniques, user-interface modules, and analyses.
developed at the Allen Institute for Brain Science and the University of North Carolina, Chapel Hill. It includes support for patch-clamp electrophysiology, multiphoton imaging, scanning laser photostimulation, and many other experimental techniques. ACQ4 is highly modular and extensible, allowing support to be added for new types of devices, techniques, user-interface modules, and analyses.
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The latest ACQ4 version is available on github: https://github.com/acq4/acq4.git For installation instructions, see the documentation older releases |
For an in-depth description of ACQ4, see our Frontiers article:
ACQ4: an open-source software platform for data acquisition and analysis in neurophysiology research.
Campagnola L, Kratz MB, and Manis PB. Front. Neuroinform., 30 January 2014 | doi: 10.3389/fninf.2014.00003
ACQ4: an open-source software platform for data acquisition and analysis in neurophysiology research.
Campagnola L, Kratz MB, and Manis PB. Front. Neuroinform., 30 January 2014 | doi: 10.3389/fninf.2014.00003
Features:
- Protocol design interface allows easy creation of stimulation / recording protocols combining and synchronizing any number of devices.
- Live camera viewing geared toward slice imaging for patch clamp and online analysis of calcium imaging.
- Scanning laser microscopy.
- Pipette automation for fast multipatch.
- Photostimulation mapping control integrated into camera/2p viewing and protocol execution.
- Integrated motion control system--all movable devices are calibrated together into one coordinate system graph.
- Online and offline data analysis system.
- Integrated data manager allows customizable data storage, browsing, and export.
- Designed to be a flexible, general-purpose experiment acquisition and analysis interface.
- Highly modular and scalable design--easy to expand support for new hardware and experiments.
- Open source and cross-platform. Runs in Windows, Linux, and OSX. (acquisition may be limited depending on your hardware, though)
Supported Hardware:
- National Instruments DAC/ADC interfaces (anything with DAQmx support, including X, S, M, and E-series)
- MultiClamp 700A, 700B
- Most scientific cameras supported via MicroManager
- Native support for Photometrics and QImaging cameras
- Scientifica microscopes and manipulators
- Sensapex uMP manipulators
- Microscope stages via MicroManager (experimental)
- Scanning galvometric mirrors (via DAC interface)
- Coherent Chameleon laser
- Most TTL-based shutters and voltage-controlled pockels cells
- Sutter MP285 and MPC-200 controllers
- AxoProbe, AxoPatch